Gel Yero Ropa Collection Tube

Kuparadzanisa gel kuunganidza ropa machubhu akashandiswa zvakanyanya mumakiriniki marabhoritari.Kupatsanura gel kunogona kuumba dura rekuzviparadzanisa pakati pezvikamu zveseru uye serum (plasma), zvinobudirira kudzivirira kuchinjanisa kwezvinhu pakati pemasero eropa neserum (plasma), uye kuve nechokwadi chekugadzikana kwezvikamu zveserum (plasma) mukati meimwe nguva yenguva.Kuparadzanisa guruu rinonyanya kuumbwa nesilicone rabha, macromolecular hydrocarbons, hydrophobic glue, etc. Sechinhu chepolymer, hachina kunyungudika mumvura uye inert.Iyo thixotropic viscous liquid ine density ye1.04-1.05 mmol/ Pakati peL, ine zvakanakira oxidation kuramba, kupisa kwakanyanya kwekushisa, kushomeka kwekushisa, uye kunaka kwemhepo.Kuwanda kweserum ndeye 1.026-1.031 mmol / L, uye hematocrit ndeye 1.090-1.095.Nekuda kweiyo giravhiti chaiyo, iyo gel inoparadzanisa ingori pakati peserum nemasero eropa, saka pasi pemamiriro ezvinhu akajairika, ropa richaonekwa mukuteerana mushure mecentrifugation.Serum, kuparadzanisa gel, uye masero eropa 3 pasi.

Kune kazhinji marudzi maviri ekuparadzanisa machubhu ekuunganidza ropa egel anowanzoshandiswa mumarabhoritari: serum separation gel procoagulation chubhu uye plasma yekuparadzanisa gel anticoagulation chubhu.Iyo serum yekuparadzanisa gel procoagulation chubhu ndeyekuwedzera coagulant kune yekuunganidza ropa chubhu kupfupisa nguva yeropa coagulation, kuwana serum nekukurumidza, uye taura mhedzisiro munguva pfupi.Girazi rekuunganidza ropa machubhu haafanire kuwedzera coagulants, uye ropa richibata girazi chubhu madziro rinokonzeresa coagulation.Nekudaro, kana coagulation zvinhu XI neXII zvasangana nemachubhu epurasitiki ekuunganidza ropa, kugona kwavo kuve activated kushomeka, uye coagulant inoda kuwedzerwa kupfupisa nguva yekubatana.Iyo plasma yekuparadzanisa gel anticoagulation chubhu inopfapfaidzwa ne anticoagulants se lithium heparin pamadziro emukati ekuparadzanisa gel yekuunganidza ropa chubhu kuzadzisa zvinodiwa nekukurumidza plasma biochemical yekuongorora emergency.

Mumashandisirwo anoshanda, zvinowanzosangana kuti kuparadzanisa mhedzisiro yekuparadzanisa gel yekuunganidza ropa chubhu haina kunaka, semuenzaniso: mune mamwe machubhu ekuparadzanisa, zvinogona kuoneka kuti kupatsanurwa kwegel zvimedu kana madonhwe emafuta ari kuyerera pamusoro pe. iyo serum kana yakamiswa mu serum;iyo yekuparadzanisa gel layer inoyangarara pane serum layer.pamusoro etc. Kuparadzanisa gels kunogonawo kukanganisa mamwe maedzo emhinduro.Mudhipatimendi redu, zvakaonekwa kuti iyo chaiyo batch reagents uye serum separation gel inomhanyisa chubhu yakabata pamwe chete panguva yeHBSAg yekuona yeAbbott i2000SR yekuongorora system, zvichikonzera mhedzisiro yenhema.

Iri pepa rinonyanya kuongorora kubva pamativi maviri, ndiko kuti, zvikonzero zvehurombo hwekuparadzanisa mhedzisiro yekuparadzanisa gel, uye pesvedzero yekuiswa kweiyo gel yekuparadzanisa pachiyero.

1. Nzira yekuparadzanisa serum uye plasma nekuparadzanisa gel Kuparadzanisa gel is thixotropic mucocolloid inoumbwa nehydrophobic organic compounds uye silica powder.Chimiro chine nhamba huru yehydrogen zvisungo.Kuvapo kwehydrogen zvisungo zvinoumba chemikemikari hwaro hwethixotropy weiyo gel inoparadzanisa..Iyo chaiyo gravity yejiri yekuparadzanisa inochengetwa pa 1.05, iyo chaiyo giravhiti yeropa remvura chikamu chinenge 1.02, uye chaiyo giravhiti yeropa rakaumbwa chikamu chinenge 1.08.Kana gel rekuparadzanisa neropa rakabatana (kana anticoagulated ropa rose) zviri centrifuged mune imwechete test tube, Nekuda kwesimba recentrifugal rinoshandiswa kune gel rinoparadzanisa, iyo hydrogen bond network yakaputsika kuita cheni-yakafanana chimiro, uye kupatsanura. gel inova yakaderera-viscosity fluid.Nekuda kwekusiyana kwakasiyana giravhiti, iyo gel yekuparadzanisa inodzoserwa kumashure uye yakamisikidzwa kuti iite matatu akaturikidzana eropa clot (anticoagulated ropa rese)/kuparadzanisa gel/serum (plasma).Kana iyo centrifuge ikamira kutenderera uye inorasikirwa ne centrifugal simba, cheni zvidimbu mugeri inoparadzanisa zvinogadzira network network zvakare nemabhondi ehydrogen, inodzoreredza yekutanga high-viscosity gel mamiriro, uye kugadzira yekuzviparadzanisa nepakati pakati peserum (plasma) uye ropa clot (anticoagulated. ropa rose)..

2. Zvikonzero zvehurombo hwekuparadzanisa mhedzisiro yekuparadzanisa gel

2.1 Kuparadzanisa Unhu hweGel Hunhu hwakananga hwejeri rekuparadzanisa huri pakati peserum (plasma) nemasero eropa, iyo ndiyo nheyo yemuviri yekudzoka kwejeri rinoparadzanisa uye kupatsanurwa kweserum (plasma).Kana kunaka kwekuparadzanisa gel yechubhu yekuunganidza ropa kwakashata uye giravhiti chaiyo isingasviki nezvinodiwa, zvichange zvichikanganisa mhedzisiro yekuparadzanisa serum (plasma), uye chiitiko chekuti gel yekuparadzanisa uye serum (plasma) ndeye. zvakabatana zvinogona kuitika.

2.2 Kusakwana kweropa coagulation Mushure me centrifugation, dzimwe nguva kupatsanurwa gel compartment uye serum uye ropa clots hazvina kuparadzaniswa zvachose, uye fibrin filaments inoonekwa muserum.Chikonzero chacho kazhinji ndechokuti ropa harina kuvharwa zvakakwana pamberi pe centrifugation.Kusakwana kweropa coagulation kunogona kukonzera kuti fibrin isanganiswe mune yekuzviparadzanisa layer.Iyo serum yekuparadzanisa rubber chubhu inofanirwa kushandiswa nemazvo maererano nemirairo, uye serum inogona kugadzirwa ne centrifugation mushure mekunge ropa razara zvachose (kazhinji, chubhu yepurasitiki ine coagulant inoda kuiswa yakatwasuka kwemaminitsi makumi matatu, uye ropa. yekuunganidza chubhu isina coagulant inoda kuiswa yakatwasuka kwe60-90 maminetsi).high-quality serum samples.

2.3 Centrifugation tembiricha Iyo tembiricha ye centrifugation ine chiitiko chakakosha pamhedzisiro yekuparadzanisa serum kubva pakuparadzanisa gel chubhu.Iyo serum yaive yakajeka mune inert yekuparadzanisa gel yakamhanyisa coagulation chubhu yakaparadzaniswa neyakajairwa centrifuge pakamuri tembiricha, asi mafuta mabhero ehukuru hwakasiyana akaonekwa mu15% kusvika 20% yemasampuli.Kune rumwe rutivi, hapana mabheji ane mafuta akawanikwa muserum akaparadzaniswa nechubhu yekuyedza centrifuged neyakadzikira tembiricha centrifuge.Kana tembiricha ichipfuura tembiricha yekuchengetedza inodiwa gel rekuparadzanisa, iyo inert gel ichanyungudika muserum.Izvo hazvingovharidzi uye kusvibisa sampuli tsono uye kapu yekuita yebiochemical analyzer, asi zvakare ine hukuru hukuru pane mamwe mhedzisiro yekuyerwa kwebiochemical.