PRP Tubes Acd Tubes
Tsanangudzo Pfupi:
Anticoagulant Citrate Dextrose Solution, inowanzonzi ACD-A kana Solution A isiri-pyrogenic, sterile solution.Ichi chinhu chinoshandiswa seanticoagulant mukugadzirwa kweplatelet-rich plasma (PRP) ine PRP Systems yeextracorporeal blood processing.
Anticoagulant Citrate Dextrose Solution, inowanzonzi ACD-A kana Solution A isiri-pyrogenic, sterile solution.Ichi chinhu chinoshandiswa seanticoagulant mukugadzirwa kweplatelet-rich plasma (PRP) ine PRP Systems yeextracorporeal blood processing.Anticoagulants ayo ari citrate-based anoshandisa kukwanisa kwecitrate ion ku chelate ionized calcium iripo muropa kudzivirira kubatanidzwa kweropa uye kuumba isina-ionized calcium-citrate complex.
Iyo chete anticoagulant chigadzirwa chakabvumidzwa neUnited States Food & Drug Administration kuti chishandiswe kugadzirira PRP mune dzakasiyana PRP Systems ndiyo ACD-A.Zvinoenderana netsvagiridzo yakaitwa muna 2016 paPRP yakawanikwa neyakasiyana-siyana anticoagulants uye mhedzisiro yavo pamaitiro e mesenchymal stromal maseru in vitro uye nhamba dzeplatelet, kune mhedzisiro yakanaka mukushandiswa kwePRP kugadzirisa musculoskeletal tissue.
Pakuparadzanisa maplatelet zvinokurudzirwa kutsiva iyo yakajairwa sodium citrate yeAcid Citrate Dextrose (ACD-A) sezvo nzira yekuzviparadzanisa inoda matanho akawanda ekugeza.Maplatelet akanyanya kugadzikana pa 37C panguva yekutenderera, asi kuatenderedza pakamuri tembiricha (25 C) inoshandawo zvakanaka.Kuderedza pH kuburikidza neACD-A (inoswedera pedyo ne6.5) kunobatsira kukanganisa kushandiswa kwezvakasara thrombin traces mumachubhu eplatelet, uye inobatsira mukuchengetwa kwese kweplatelet morphology uchishandura basa kune minima.Kazhinji iwe unofanirwa kumisazve maplatelet kune yakakodzera Tyrode Buffer (pH 7.4) kuitira kuti adzore kushanda kwawo.ACD ine zvakawanda zvakanaka kana zvasvika pakuchengetedza maplatelet
Apo ACD yakashandiswa, zvigumisiro zvakaratidza goho repamusoro replatelet muropa rose.Zvisinei, kushandiswa kweEDTA kwakakurudzirawo kukura muhuwandu hweplatelet volume mushure mekunge ropa centrifugation matanho akaitwa kuti awane PRP.Zvadaro, kushandiswa kweACD kwakaguma nekuwedzera kuwanda kwemaseru stromal masero.Naizvozvo, zvakagumiswa kuti maanticoagulants anosanganisira ACD-A anoita basa rakakura mukugadzirira kwePRP uye anobatsira kukwirisa maitiro kusvika pamwero mukuru.
