Nucleic Acid Detection White Tube
Tsanangudzo Pfupi:
Inonyanya kushandiswa kuona nucleic acid, uye inogadzirwa zvizere pasi pemamiriro ekucheneswa, izvo zvinoderedza kusvibiswa kunogona kuitika panguva yekugadzira uye nekudzikisa zvinobudirira kukanganisa kwekugona kutakura-pamusoro kusvibiswa pakuedza.
1. Suction volume experiment: Kusveta vhoriyamu, ndiko kuti, kuwanda kweropa rakatorwa, ine kukanganisa mukati me ± 10%, kana zvisina kudaro haina kukodzera.Huwandu husina kururama hweropa rakatorwa ndiro dambudziko guru pari zvino.Izvi hazvingogumiri pamigumisiro yekuongorora isina kururama, asiwo inokonzera kuvhara uye kukanganisa kumidziyo yekuongorora.
2. Container leakage experiment: Iyo vacuum yekuunganidza ropa chubhu ine sodium fluorescein composite solution yakaiswa yakatarisa pasi mumvura ine deionized kwemaminetsi makumi matanhatu.Pasi peiyo refu-wave ultraviolet mwenje sosi, hapana fluorescence yakaonekwa pasi pechiono chakajairwa muimba yerima, iyo yaikwanisa.Kubvinza kwemudziyo ndicho chikonzero chikuru chehuwandu hweropa husina kujeka hweiyo ikozvino vacuum yekuunganidza ropa chubhu.
3. Kuedzwa kwesimba remudziyo: mudziyo unoiswa pasi pe centrifuge ine centrifugal acceleration ye 3000g kwemaminitsi gumi, uye inokodzera kana ikasaputika.Zvinetso zvinodikanwa kunze kwenyika ndezvi: 2 metres pamusoro pevhu, iyo vacuum yekuunganidza ropa chubhu inodonha yakatwasuka isingatyoke, iyo inogona kudzivirira netsaona kukuvara kweye test chubhu uye kurasikirwa kwemuenzaniso.
4. Kuyedza nzvimbo shoma yemahara: Nzvimbo shoma yekuona kuti ropa ranyatsosanganiswa.Kuwanda kweropa rakatorwa 0.5ml-5ml, > + 25% yehuwandu hweropa rakatorwa;kana huwandu hweropa hwakatorwa huri > 5ml, > 15% yehuwandu hweropa rakatorwa.
5. Kunyatsoedza kuedza kwe solvent, solute mass ratio uye mhinduro yekuwedzera huwandu: mhosho inofanira kunge iri mukati ± 10% yezvakatsanangurwa chiyero chirimwa.Iri idambudziko riri nyore kufuratirwa uye rinowanzoitika, uye ndicho chimwe chezvikonzero chikuru che data rebvunzo risiri chairo.
