Platelet-rich plasma (PRP) ndeye autologous concentration yemaplatelet emunhu muplasma.Kuburikidza nekuderera kwealpha granules mumaplatelet, PRP inogona kuvhara zvinhu zvakasiyana-siyana zvekukura, kusanganisira platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), hepatocyte growth factor (HGF), uye kushandura. kukura chinhu (TGF), iyo yakanyorwa kuti itange kuporesa maronda uye kukurudzira kupararira uye kuchinjwa kwemasero ekupedzisira uye pericytes kuva endothelial sprouts.
Mabasa ePRP ekurapa kwebvudzi kukura akashumwa mune dzakawanda dzichangobva kutsvagurudzo.Uebel et al.vakawana kuti platelet plasma kukura zvinhu zvinowedzera goho refollicular units mumurume maitiro ekuvhiya mhanza.Basa richangoburwa rakaratidza kuti PRP inowedzera kuwanda kwedermal papilla masero uye kukurudzira nekukurumidza telogen-to-anagen shanduko uchishandisa mu vivo uye in vitro modhi.Imwe ongororo yakaratidza kuti PRP inokurudzira kudzoreredzwa kwebvudzi rebvudzi uye kupfupisa zvakanyanya nguva yekuumbwa kwebvudzi.
Zvose PRP uye platelet-varombo plasma (PPP) zvinosanganisira kukwana kwakakwana kweprotein coagulation.Muchidzidzo chezvino, pesvedzero yePRP nePPP pakukura kwebvudzi muC57BL/6 mice yakaongororwa.Iko kufungidzira kwaiva kuti PRP yaiva nemigumisiro yakanaka pakureba kwebvudzi kukura uye kuwedzera kwenhamba yebvudzi follicles.
Mhuka dzekuedza
Akazara makumi mashanu ane hutano C57BL/6 mbeva dzechirume (masvondo matanhatu, 20 ± 2 g) dzakawanikwa kubva kuCentre of Laboratory Animals, Hangzhou Normal University (Hangzhou, China).Mhuka dzaidyiswa zvokudya zvakafanana uye dzaichengetwa munzvimbo isingachinji pasi pe 12:12-h light-dark cycle.Mushure mevhiki ye1 yeacclimatization, mbeva dzakakamurwa zvisina tsarukano kuita mapoka matatu: PRP boka (n = 10), PPP boka (n = 10), uye boka rekutonga (n = 10).
Protocol yekudzidza yakatenderwa nekomiti yezvetsika yekuongorora mhuka pasi peMutemo weKutsvagisa Mhuka uye Mitemo yemitemo muChina.
Kuyera kureba kwebvudzi
Pa8, 13, uye mazuva 18 mushure mejekiseni rekupedzisira, vhudzi gumi mumutsara wega wega dzakasarudzwa munzvimbo yaitarisirwa.Kuyera kureba kwebvudzi kwakaitwa muminda mitatu vachishandisa maikorosikopu erekitironi, uye avhareji yavo yairatidzwa semamirimita.Mvere dzakarebesa kana dzakakuvara dzaisabatanidzwa.
Hematoxylin uye eosin (HE) tsvina
Dorsal skin samples yakabviswa pamazuva gumi nemasere mushure mejekiseni rechitatu.Ipapo samples dzakagadziriswa mu 10% isina kwayakarerekera buffered formalin, yakaiswa muparafini, uye yakachekwa kuita 4 μm.Zvikamu zvakabikwa kwe4 h kuitira deparaffinization pa65 ° C, yakanyikwa mu gradient ethanol, uyezve yakasvibiswa nehematoxylin kwemaminetsi mashanu.Mushure mekusiyaniswa mu1% hydrochloric acid doro, zvikamu zvakaputirwa mumvura yeammonia, yakasvibiswa neeosin, uye yakashambidzwa nemvura yakasvibiswa.Pakupedzisira, zvikamu zvakasvibiswa ne gradient ethanol, yakacheneswa nexylene, yakasungirirwa neine neutral resin, uye yakacherechedza kushandisa microscope (Olympus, Tokyo, Japan).
Nguva yekutumira: Oct-12-2022